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AMACR Antibody

Rabbit Monoclonal Antibody

  • WB - AMACR Antibody AJ1035b
    Western blot analysis on (A) LNCaP (B) HepG2 cell lysate (C) Mouse heart (D) Mouse kidney and (E) Rat kidney lysates using anti-AMACR RabMAb (Cat. #AJ1035b).
  • IHC - AMACR Antibody AJ1035b
    Immunohistochemical analysis of paraffin-embedded human prostatic adenocarcinoma using antyi-AMACR RabMAb (Cat. #AJ1035b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UHK6
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3717
Calculated MW 42387 Da
Gene ID 23600
Other Names Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase, AMACR
Target/Specificity A synthetic peptide corresponding to residues in human AMACR was used as an immunogen.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAMACR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
Cellular Location Peroxisome. Mitochondrion
Research Areas
Citations (0)

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Alpha-methylacyl-CoA racemase (AMACR) primary role involves the racemization of 2-methyl-branched fatty acid CoA esters and the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers (1). A central role in all the metabolic pathways is played by AMACR, which regulates metabolism of lipids. AMACR regulates the entry of branched-chain lipids into the peroxisomal and mitochondrial beta-oxidation pathways (2). It has also been proven to be one of the few biomarkers that can help distinguish cancer from benign cells, with high sensitivity and specificity for prostate carcinoma (3). Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) that results in elevated plasma concentrations of pristanic acid C27-bile-acid intermediates. It can be associated with polyneuropathy, retinitis pigmentosa and epilepsy (1).


1. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195 (2008) 2. Lloyd MD, et al. FEBS J. 275(6):1089-102, 2008 3. Jiang Z, et al. Am J Clin Pathol. 122(2):275-89, 2004

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Cat# AJ1035b
(40 western blots)
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