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Amphiphysin Antibody

Rabbit Monoclonal Antibody

  • WB - Amphiphysin Antibody AJ1036a
    A. Western blot analysis on human brain lysate using anti-Amphipysin RabMAb (Cat. #AJ1036a), dilution 1:100,000.
  • IHC - Amphiphysin Antibody AJ1036a
    B. Immunohistochemical analysis of paraffin-embedded human brain using anti-Amphiphysin RabMAb (Cat. #AJ1036a).
  • IF - Amphiphysin Antibody AJ1036a
    C. Immunofluorescent staining of SH-SYS5 cells using anti-Amphiphysin RabMAb (Cat. #AJ1036a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49418
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP2060Y
Calculated MW 76257 Da
Gene ID 273
Other Names Amphiphysin, AMPH, AMPH1
Target/Specificity A synthetic peptide corresponding to residues within human Amphipysin was used as an immunogen.
Dilution WB~~1:50000~100000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAmphiphysin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms AMPH1
Function May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Cellular Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton
Tissue Location Neurons, certain endocrine cell types and spermatocytes
Research Areas
Citations (0)

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Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).


1. David C, et al. FEBS Lett 351(1):73-9, 1994
2. Yamamoto R, et al. Hum Mol Genet 4(2):265-8, 1995
3. Floyd S, et al. Mol Med 4(1):29-39, 1998

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Cat# AJ1036a
(40 western blots)
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