|Application ||IHC, WB|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||76257 Da|
|Other Names||Amphiphysin, AMPH, AMPH1|
|Target/Specificity||A synthetic peptide corresponding to residues near C-terminus of human Amphipysin was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Amphiphysin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.|
|Cellular Location||Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton|
|Tissue Location||Neurons, certain endocrine cell types and spermatocytes|
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Provided below are standard protocols that you may find useful for product applications.
Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).
1. David C, et al. FEBS Lett 351(1):73-9, 1994
2. Yamamoto R, et al. Hum Mol Genet 4(2):265-8, 1995
3. Floyd S, et al. Mol Med 4(1):29-39, 1998
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