|Application ||WB, IHC|
|Calculated MW||141840 Da|
|Other Names||Apoptotic protease-activating factor 1, APAF-1, APAF1, KIAA0413|
|Target/Specificity||A synthetic peptide corresponding to residues near N-terminus of human Apaf-1 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Apaf-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.|
|Tissue Location||Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Apaf-1, or apoptotic protease activating factor 1, is a human protein homolog of the C. elagans apoptotic regulatory protein CED-4 that activates caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms an oligomeric complex with caspase-9 in the presence of cytochrome c and dATP, which leads to caspase-9 activation and subsequent activation of caspase-3 (2, 3). Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains, consequently enhancing the catalytic activity of caspase-9 (4), leading to activation of the caspase signaling cascade and apoptosis.
1. Zou, H. et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90(3): 405
2. Saleh, A. et al. Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation. J. Biol. Chem. 274 (25): 17941
3. Hu, Y. et al. Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis. EMBO J. 18(13): 3586
4. Shiozaki, E.N. et al. Oligomerization and activation of caspase-9, induced by Apaf-1 CARD. PNAS 99(7): 4197
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.