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Apaf-1 Antibody

Rabbit Monoclonal Antibody

     
  • WB - Apaf-1 Antibody AJ1048a
    A. Western blot analysis on HeLa cell lysate using anti-Apaf-1 RabMAb (Cat. #AJ1048a); dilution 1:500.
  • IHC - Apaf-1 Antibody AJ1048a
    B. Immunohistochemical analysis of paraffin-embedded stomach adenocarcinoma using anti-Apaf-1 RabMAb (Cat. #AJ1048a).
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession O14727
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names E38
Calculated MW 141840 Da
Gene ID 317
Other Names Apoptotic protease-activating factor 1, APAF-1, APAF1, KIAA0413
Target/Specificity A synthetic peptide corresponding to residues near N-terminus of human Apaf-1 was used as immunogen.
Dilution IHC~~1:50~1:100
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsApaf-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APAF1
Synonyms KIAA0413
Function Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Cellular Location Cytoplasm.
Tissue Location Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver
Research Areas
Citations ( 0 )

Background

Apaf-1, or apoptotic protease activating factor 1, is a human protein homolog of the C. elagans apoptotic regulatory protein CED-4 that activates caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms an oligomeric complex with caspase-9 in the presence of cytochrome c and dATP, which leads to caspase-9 activation and subsequent activation of caspase-3 (2, 3). Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains, consequently enhancing the catalytic activity of caspase-9 (4), leading to activation of the caspase signaling cascade and apoptosis.

References

1. Zou, H. et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90(3): 405
2. Saleh, A. et al. Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation. J. Biol. Chem. 274 (25): 17941
3. Hu, Y. et al. Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis. EMBO J. 18(13): 3586
4. Shiozaki, E.N. et al. Oligomerization and activation of caspase-9, induced by Apaf-1 CARD. PNAS 99(7): 4197

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Discontinued
Cat# AJ1048a
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