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Apolipoprotein J Antibody

Rabbit Monoclonal Antibody

  • WB - Apolipoprotein J Antibody AJ1054a
    Western blot analysis on (A) Human tonsil tissue (B) fetal brain and (C) human plasma lysate using anti-ApoJ RabMAb (Cat. #AJ1054a).
  • IHC - Apolipoprotein J Antibody AJ1054a
    Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ApoJ RabMAb (Cat. #AJ1054a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10909
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR2911
Calculated MW 52495 Da
Gene ID 1191
Other Names Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Apo-J, Complement cytolysis inhibitor, CLI, Complement-associated protein SP-40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate message 2, TRPM-2, Clusterin beta chain, ApoJalpha, Complement cytolysis inhibitor a chain, Clusterin alpha chain, ApoJbeta, Complement cytolysis inhibitor b chain, CLU, APOJ, CLI, KUB1
Target/Specificity A synthetic peptide corresponding to residues in the alpha chain of human ApoJ was used as an immunogen.
Dilution WB~~1:1000~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsApolipoprotein J Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CLU
Synonyms APOJ, CLI, KUB1
Function Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
Cellular Location Isoform 1: Secreted. Note=Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress
Tissue Location Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung
Research Areas
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Apolipoprotein J (ApoJ) is a 70-kD protein associated with high-density lipoproteins (HDL) in human plasma. The protein is synthesized as a 427-amino acid polypeptide that is posttranslationally cleaved at an internal bond between arg205 and ser206. Two subunits, designated alpha (34 to 36 kD), corresponding to residues 1-205, and beta (36 to 39 kD), corresponding to residues 206-427, are associated through disulfide bonds (1). ApoJ is a stress-induced cytoprotective chaperone protein, regulated by HSF1, and functions similarly to a small heat-shock protein. ApoJ is expressed in a variety of cancers and associated with broad-spectrum treatment resistance (2).


1. de Silva HV, et al. J Biol Chem 265 :14292-14297, 1990 2. Chi KN, et al. Expert Opin Investig Drugs 17(12) :1955-62, 2008

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Cat# AJ1054a
(40 western blots)
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