|Calculated MW||151423 Da|
|Other Names||Partitioning defective 3 homolog, PAR-3, PARD-3, Atypical PKC isotype-specific-interacting protein, ASIP, CTCL tumor antigen se2-5, PAR3-alpha, PARD3, PAR3, PAR3A|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human ASIP was used as immunogen. This antibody is predicted to detect isoforms 2-7 based on sequence analysis.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ASIP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.|
|Cellular Location||Cytoplasm. Endomembrane system. Cell junction. Cell junction, tight junction. Cell membrane. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Note=Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II Colocalized with SIRT2 in internode region of myelin sheat (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
The agouti signaling protein (ASIP) is an adaptor protein involved in asymmetrical cell division and cell polarization processes. ASIP seems to play a central role in the formation of epithelial tight junctions. ASIP may affect the quality of hair pigmentation, and act as a pharmacological antagonist of alpha-melanocyte-stimulating hormone (2-3). ASIP may also play a role in neuroendocrine aspects of melanocortin action, and have a functional role in regulating lipid metabolism in adipocytes (4-5).
1. Kwon, et al Proc. Nat. Acad. Sci. 91: 9760-9764, 1994.
2. Hu Y, Fang C, Xu Y.2005, Cell Mol Life Sci. Sep;62(17):1974-83.
3. Bonilla et al.2005 Hum Genet. Apr;116(5):402-6. Epub 2005 Feb 22.
4. Landi et al. 2005 J Natl Cancer Inst. Jul 6;97(13):998-1007.
5. Smith et al. Diabetes. 2003 Dec;52(12):2914-22.
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