|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||64370 Da|
|Other Names||Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase, ASNS, TS11|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human asparagine synthetase was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Asparagine-Synthase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Asparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells, in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines (1). Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia (2).
1. Gantt, J. S., C.-S. Chiang, G. W. Hatfield, and S. M. Arfin. J. Biol. Chem. 255:4808-4813.
2. Ortega, J. A., M. E. Nesbit, Jr., M. H. Donaldson, R. H. Hittle, J. Weiner, M. Karon, and D. Hammond. 1977. Cancer Res. 37:535-540.
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