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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Asparagine-Synthase Antibody   

Asparagine-Synthase Antibody

Rabbit Monoclonal Antibody

  • WB - Asparagine-Synthase Antibody AJ1062a
    A. Western blot analysis on K562 cell lysate using anti-Asparagine Synthetase RabMAb (Cat. #AJ1062a), dilution 1:2,000.
  • IHC - Asparagine-Synthase Antibody AJ1062a
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Asparagine Synthetase RabMAb (Cat. #AJ1062a)
  • IHC - Asparagine-Synthase Antibody AJ1062a
    C. Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-Asparagine Synthetase RabMAb (Cat. #AJ1062a)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08243
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP282Y
Calculated MW 64370 Da
Gene ID 440
Other Names Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase, ASNS, TS11
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human asparagine synthetase was used as an immunogen.
Dilution WB~~1:10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAsparagine-Synthase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms TS11
Research Areas
Citations (0)

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Asparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells, in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines (1). Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia (2).


1. Gantt, J. S., C.-S. Chiang, G. W. Hatfield, and S. M. Arfin. J. Biol. Chem. 255:4808-4813.
2. Ortega, J. A., M. E. Nesbit, Jr., M. H. Donaldson, R. H. Hittle, J. Weiner, M. Karon, and D. Hammond. 1977. Cancer Res. 37:535-540.

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Cat# AJ1062a
(40 western blots)
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