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ATF-1 Antibody Phospho (pS63)

Rabbit Monoclonal Antibody

  • WB - ATF-1 Antibody Phospho (pS63) AJ1063a
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-ATF-1 (pS63) RabMAb (Cat. #AJ1063a), 1:100,000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase.
  • IHC - ATF-1 Antibody Phospho (pS63) AJ1063a
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-specific anti-ATF-1 RabMAb (Cat. #AJ1063a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P18846
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP1591(2)Y
Calculated MW 29233 Da
Gene ID 466
Other Names Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1, Protein TREB36, ATF1
Target/Specificity A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF-1 was used as an immunogen. This antibody detects ATF-1 phosphorylated on serine 63
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATF-1 Antibody Phospho (pS63) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATF1
Function This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
Cellular Location Nucleus.
Research Areas
Citations (0)

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ATF-1 is a cAMP-responsive enhancer (CRE) binding protein that is expressed in a wide variety of cell types and is capable of dimerizing with CREB. ATF-1 contains a consensus phosphorylation site for PKA and shares extensive homology with CREB in the region surrounding and carboxyl-terminal to the PKA site (1). Epidermal growth factor induction of c-jun expression requires ATF-1 and MEF2 sites in the c-jun promoter. Activation of the c-jun promoter through the ATF-1 site requires phosphorylation of ATF-1 at serine 63. Two MAPK pathways, ERK and JNK, are required for EGF-induced c-jun expression and the ERK pathway acts through downstream phosphorylation of ATF-1 (2).


1. Rehfuss RP, et al J Biol Chem 266(28):18431-4, 1991
2. Gupta P et al. J Biol Chem 277(52):50550-6, 2002

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Cat# AJ1063a
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