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ATM Antibody

Rabbit Monoclonal Antibody

  • WB - ATM Antibody AJ1066a
    A. Western blot analysis on 293 cell lysate using anti-ATM RabMAb (Cat. #AJ1066a) dilution 1:5,000.
  • IHC - ATM Antibody AJ1066a
    B. Immunohistochemical analysis of paraffin-embedded human carcinoma using anti-ATM RabMAb (Cat. #AJ1066a).
  • IF - ATM Antibody AJ1066a
    C. Immunofluorescent staining of 293 cells using anti-ATM RabMAb (Cat. #AJ1066a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13315
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names Y170
Calculated MW 350687 Da
Gene ID 472
Other Names Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATM
Target/Specificity A synthetic peptide corresponding to residues surrounding Serine 1981 of human ATM was used as immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATM
Function Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.
Cellular Location Nucleus. Cytoplasmic vesicle. Note=Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin
Tissue Location Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes
Research Areas
Citations (0)

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ATM is a nuclear phosphoprotein involved in Ataxia-telangiectasia (A-T) an autosomal recessive disorder characterized by cerebellar ataxia, immune deficiencies, increased cancer predisposition, chromosomal instability and radiation sensitivity (1-2). This large protein (370 kDa) is involved in genome stability, cellular responses to DNA damage and cell cycle control. More than 100 mutations have been identified so far and are expected to inactivate the ATM protein by truncation or large deletions (3). Defects in ATM contribute to various B cell and T cell Leukemia (4).


1. Harnden, D.G. (1994) Int. J. Radiat. Biol. 66, S13-S19
2. Lavin, M.F. and Shiloh, Y. (1997) Ann. Rev. Immunol. 15, 177-202
3. Savitsky, K., Sfez, S., Tagle, D.A., Ziv, Y., Sartiel, A., Collins, F.S., Shiloh, Y. and Rotman, G. (1995) Hum. Mol. Gen. 4, 2025-2032.
4. Stankovic T., Weber P., Stewart G., Bedenham T., Murray J., Byrd P.J., Moss P.A.H., Taylor A.M.R.; Lancet 353:26-29(1999).

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Cat# AJ1066a
(40 western blots)
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