|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||120839 Da|
|Other Names||ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, ACL, Citrate cleavage enzyme, ACLY|
|Target/Specificity||A synthetic phospho-peptide corresponding to residues surrounding Thr447 and Ser451 of human ATP-citrate (pro-S) lyase was used as immunogen. The antibody only detects ATP-citrate (pro-S) lyase phosphorylated on Threonine 447 and Serine 451.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATP-citrate lyase Antibody Phospho (pT447/pS451) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.|
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Provided below are standard protocols that you may find useful for product applications.
ATP citrate lyase (ACLY), also known as ATP citrate synthase, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACLY and insulin to increase phosphorylation of ACLY (2).
1. Elshourbagy et al. Europ. J. Biochem. 204: 491-499, 1992.
2. Benjamin, W. B., and Singer, I. (1974) Biochim. Biophys. Acta 251, 28-422.
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