|Application ||WB, IHC|
|Calculated MW||45809 Da|
|Other Names||Aurora kinase A, Aurora 2, Aurora/IPL1-related kinase 1, ARK-1, Aurora-related kinase 1, hARK1, Breast tumor-amplified kinase, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, AURKA|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human Aurora-A was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Aurora-A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Detected at the neurite hillock in developing neurons (By similarity). Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase. Colocalized with SIRT2 at centrosome. Moves to the midbody during both telophase and cytokinesis. Associates with both the pericentriolar material (PCM) and centrioles.|
|Tissue Location||Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Aurora-A is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process (1). Aurora-A is ubiquitously expressed and activated through an interaction with Ajuba and TPX2 during late G2 and mitotic phases. Activated form regulates the functions of centrosomes, spindles, and kinetochores required for normal mitotic progression. Initially localized at centrosome during interphase, Aurora-A is translocated to mitotic spindles in early mitotic phase and finally degraded after metaphaseanaphase transition (2). Overexpression of Aurora-A is seen various tumors, where an overexpression can induce abnormalities in G2 and Spindle checkpoints, and cytokinesis failure (3).
1. Katayama H, et al. Cancer Metastasis Rev. 22(4): 451-64, 2003
2. Duct D et al, Exp Cell Res. 15;301(1):60-7, 2004.
3. Murimoto T et al, Nat Rev Cancer. Jan;5(1):42-50, 2005.
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