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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   B7-2/CD86 Antibody   

B7-2/CD86 Antibody

Rabbit Monoclonal Antibody

  • WB - B7-2/CD86 Antibody AJ1072a
    A. Western blot analysis on Raji cell lysate using anti-B7-2 RabMAb (Cat. #AJ1072a), dilution 1:5000
  • IHC - B7-2/CD86 Antibody AJ1072a
    B. Immunohistochemical staining of paraffin-embedded human tonsils using anti-B7-2 RabMAb (Cat. #AJ1072a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P42081
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP1158Y
Calculated MW 37682 Da
Gene ID 942
Other Names T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B72, FUN-1, CD86, CD86, CD28LG2
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human B7-2 was used as an immunogen.
Dilution WB~~1:10000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB7-2/CD86 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD86
Synonyms CD28LG2
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Expressed by activated B-lymphocytes and monocytes
Research Areas
Citations (0)

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B7-2 (CD86) is an important costimulatory molecule for the priming and activation of naive and memory T cells, respectively. Soluble CD86 is produced by resting monocytes and results from an alternatively spliced transcript (CD86deltaTM) characterized by deletion of the transmembrane domain (1). Engagement of CD28 with B7-1 and B7-2 ligands on antigen-presenting cells (APCs) provides a stimulatory signal for T-cell activation, whereas subsequent engagement of CTLA-4 with these same ligands results in attenuation of the response. Given their central function in immune modulation, CTLA-4- and CD28-associated signaling pathways are primary therapeutic targets for preventing autoimmune disease, graft versus host disease, graft rejection and promoting tumor immunity (2). It has also been shown that on B cells and macrophages, heat shock proteins GroES and GroEL both stimulated the expression of B7-2 (3)


1. Jeannin P, et al. Immunity 13(3):303-12, 2000.
2. Schwartz JC, et al. Nature 410(6828):604-8, 2001.
3. Galdiero M, et al. Int. J Immunopathol Pharmacol 18(4):637-44, 2005.

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Cat# AJ1072a
(40 western blots)
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