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B7-H4 Antibody

Rabbit Monoclonal Antibody

  • WB - B7-H4 Antibody AJ1073a
    Western blot analysis on A) fetal spleen B) fetal thymus, C) SKBR-3 lysates using anti-B7-H4 RabMAb (Cat. #AJ1073a), 1:10,000 dilution.
  • IHC - B7-H4 Antibody AJ1073a
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-B7-H4 RabMAb (Cat. #AJ1073a)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7Z7D3
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP1165
Calculated MW 30878 Da
Gene ID 79679
Other Names V-set domain-containing T-cell activation inhibitor 1, B7 homolog 4, B7-H4, B7h5, Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, VTCN1 {ECO:0000312|EMBL:EAW566721}
Target/Specificity A peptide corresponding to residues near N-term of human GNB2 was used as an immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB7-H4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VTCN1 {ECO:0000312|EMBL:EAW56672.1}
Function Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
Cellular Location Cell membrane; Single-pass type I membrane protein. Note=Expressed at the cell surface. A soluble form has also been detected. {ECO:0000255, ECO:0000269|PubMed:12818165, ECO:0000269|PubMed:15878339, ECO:0000269|PubMed:16782226}
Tissue Location Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC) Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis.
Research Areas
Citations (0)

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As a member of B7 family, B7-H4 (VTCN1) plays a critical role in the regulation of antigen-specific immune responses. B7-H4 is a co-stimulatory protein expressed in activated T cells and is involved in the negative regulation of cell-mediated immunity in peripheral tissues (1). B7-H4 ligation to its receptor BTLA T-lymphocytes results in inhibition of T-cell activation, cytokine secretion, and the development of cytotoxicity (2). B7-H4 protein is typically expressed on the surface of activated T-cells, B-cells, monocytes, and dendritic cells, but not in most normal tissues. In tissues, B7-H4 is over expressed in breast, endometrial, ovarian, renal cell (RCC), and non-small cell lung carcinomas (NSCLC). B7-H4 has been identified as an important cancer marker for breast and ovarian cancers (3).


1. Tringler B, et al.,Clinical Cancer Research 11:1842-1848, 2005. 2. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195(2008). 3. Simon I, et al., Cancer Research 66:1570 - 1575, 2006.

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Cat# AJ1073a
(40 western blots)
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