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Bag-1 Antibody

Rabbit Monoclonal Antibody

  • WB - Bag-1 Antibody AJ1075a
    A. Western blot analysis on HeLa cell lysate using anti-Bag-1 RabMAb (Cat. #AJ1075a). Dilution 1:500
  • IHC - Bag-1 Antibody AJ1075a
    B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-Bag-1 RabMAb (Cat. #AJ1075a).
  • IF - Bag-1 Antibody AJ1075a
    C. Immunofluorescent stsining of HeLa cells using anti-Bag-1 RabMAb (Cat. #AJ1075a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99933
Reactivity Human, Rat
Host Rabbit
Clonality Monoclonal
Clone Names Y166
Calculated MW 38779 Da
Gene ID 573
Other Names BAG family molecular chaperone regulator 1, BAG-1, Bcl-2-associated athanogene 1, BAG1, HAP
Target/Specificity A synthetic peptide corresponding to residues following the repeat 7 of human Bag-1 was used as immunogen. The antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S).
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBag-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BAG1
Synonyms HAP
Function Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
Cellular Location Isoform 1: Nucleus. Cytoplasm. Note=Isoform 1 localizes predominantly to the nucleus Isoform 4: Cytoplasm. Nucleus. Note=Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria
Tissue Location Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level)
Research Areas
Citations (0)

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Members of the Bcl-2 family of proteins play varied stimulatory or inhibitory roles in the regulation of programmed cell death, or apoptosis. One member of this family, Bcl2-Interacting Protein-1 (BAG-1), functions in conjunction with Bcl-2 to inhibit apoptosis (1,2). BAG-1 is a heat shock 70 kDa (Hsp70)- binding protein (3) that is localized to either the cytosol, nucleus or both.


1. Takayama, S. et al. Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12. Genomics 35: 494
2. Matsuzawa, S. et al. p53-inducible human homologue of Drosophila seven in absentia (Siah) inhibits cell growth: suppression by BAG-1. EMBO J. 17: 2736
3. Takayama, S. et al. An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators. J Biol Chem. 274: 781

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Cat# AJ1075a
(40 western blots)
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