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YWHAE Antibody

Rabbit Monoclonal IgG

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United States
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Ordering Information
Catalog # SizeAvailability Price  
AJ1077a 100µl 2-3 days
$ 295.00email & fax$ 280.25online
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YWHAE Antibody - Product Information

Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary AccessionO95817
ReactivityHuman
Clone NamesEPR3515
Calculated MW61595 Da
Gene ID 9531
Target/Specificity
A synthetic peptide corresponding to residues in human Bag-3 was used as an immunogen.
Dilution
WB~~1:1000~10000
Format
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
YWHAE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

YWHAE Antibody - Protein Information

Name BAG3
Synonyms BIS
Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.

YWHAE Antibody - Related Products

AT1262a: BAG3 Antibody (monoclonal) (M01)

AP50650: BAG3 Antibody

AF2265a: BAG3 / BIS/ CAIR1 Antibody (C-Term)

AF2638a: BAG3 Antibody (internal region)

YWHAE Antibody - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Bcl-2-associated athanogene 3 (Bag-3) is a member of a conserved family of cyto-protective proteins that bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone acitivity in a Hip-repressible manner. It has been shown to be prominently expressed in striated muscle and colocalizes with Z-disks. Bag-3 is not required for muscle development, but appears to be critically important for maintenance of mature skeletal muscle (1-2). Bag-3 has also been shown to have anti-apoptotic activity (3). Overexpression of Bag-3 has prevented or reduced the distraction of polyubiquitinated Hsp90/Hsp70 client proteins such as Cyclin D1, Akt, glycogen synthase 3B, and p70s6 kinase, and facilitated cell survival (4).

REFERENCES

1. Homma S, et al. Am J Pathol. 169(3):761-73, 2006 2. NCBI Reference Sequence (RefSeq): a curated non- redundant sequence database of genomes, transcripts and proteins Pruitt KD, Tatusova, T, Maglott DR Nucleic Acids Res 2007 Jan 1;35(Database issue):D61-5 3. The UniProt Consortium. The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2008) 4. Virador VM., et al. PLoS One 4(4):e5136, 2009