|Application ||WB, IHC|
|Calculated MW||23409 Da|
|Other Names||Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-term of human Bak was used as immunogen. The antibody does not cross-react with other Bcl-2 members.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Bak Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BAK, BCL2L7, CDN1|
|Function||Plays a role in the mitochondrial apoptosic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.|
|Cellular Location||Mitochondrion membrane; Single-pass membrane protein|
|Tissue Location||Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
Bak a pro-apoptotic analog to Bax, is a member of the Bcl-2 family of proteins. During mitochondria-regulated programmed cell death, Bak promotes apoptosis by binding Bcl-2 and inhibiting its anti-apoptotic function (1). Upon apoptotic stimulation, Bak translocates from the cytosol to the mitochondrial membrane (2). Pro-apoptotic cascade activates BID, which oligomerizes Bak into pores that result in the release of cytochrome c (2, 3). In healthy cells, Bak associate with Bcl-xL and Mcl-1 but not with Bcl-2, Bcl-w or A1 and only when freed of both Bak will induce apoptosis.
1. Greider, C.W., and Blackburn, E.H. (1985). Cell 43, 405-413.
2. Feng J, Funk WD, Wang SS, et al. Science 1995; 269:1236-41.
3. Greider, C.W. (1996). Telomere length regulation. Annu. Rev. Biochem. 65, 337-365.
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