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Bak AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1078a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Bak Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q16611 |
| Reactivity | Human |
| Clone Names | Y164 |
| Calculated MW | 23409 Da |
| Gene ID 578 | |
| Other Names BAK1, BAK, BCL2L7, CDN1, Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK;Bcl-2-like protein 7 | |
| Target/Specificity A synthetic peptide corresponding to residues near the N-term of human Bak was used as immunogen. The antibody does not cross-react with other Bcl-2 members. | |
| Dilution WB~~1:5000~10000 IHC~~1:250~500 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Bak Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Bak Antibody - Protein Information | |
| Name BAK1 | |
| Synonyms BAK, BCL2L7, CDN1 | |
| Function In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis | |
| Cellular Location Mitochondrion membrane; Single-pass membrane protein (Potential) | |
| Tissue Location Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle | |
Bak Antibody - Related products
AP1301a: Bak Antibody (BH3 Domain Specific)
LY11623a: BAK1 Over-expression Lysate
Bak Antibody - Application data
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A. Western blot analysis on Hela cell lysate using anti-Bak RabMAb (Cat. #AJ1078a). dilution 1:5,000.
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B. Immunohistochemical analysis of paraffin-embedded human stomack carcinoma using anti-Bak RabMAb (Cat. #AJ1078a).
Bak Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Bak a pro-apoptotic analog to Bax, is a member of the Bcl-2 family of proteins. During mitochondria-regulated programmed cell death, Bak promotes apoptosis by binding Bcl-2 and inhibiting its anti-apoptotic function (1). Upon apoptotic stimulation, Bak translocates from the cytosol to the mitochondrial membrane (2). Pro-apoptotic cascade activates BID, which oligomerizes Bak into pores that result in the release of cytochrome c (2, 3). In healthy cells, Bak associate with Bcl-xL and Mcl-1 but not with Bcl-2, Bcl-w or A1 and only when freed of both Bak will induce apoptosis.
REFERENCES
1. Greider, C.W., and Blackburn, E.H. (1985). Cell 43, 405-413.
2. Feng J, Funk WD, Wang SS, et al. Science 1995; 269:1236-41.
3. Greider, C.W. (1996). Telomere length regulation. Annu. Rev. Biochem. 65, 337-365.
