|Application ||WB, IHC, IF|
|Calculated MW||20132 Da|
|Other Names||Bcl-2-related protein A1, Bcl-2-like protein 5, Bcl2-L-5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS, BCL2A1, BCL2L5, BFL1, GRS, HBPA1|
|Target/Specificity||A synthetic peptide corresponding to residues in the N-term of human Bcl-2 related protein A1 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Bcl-2 A1/Bfl1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BCL2L5, BFL1, GRS, HBPA1|
|Function||Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection (By similarity).|
|Tissue Location||Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies|
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Provided below are standard protocols that you may find useful for product applications.
Bcl-2 related protein A1; also known as Bcl2A1 and Bfl1, is an anti-apoptotic Bcl-2 family member preferentially expressed in hematopoietic and endothelial cells (1). Bfl1 is involved in a variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. Expression of Bfl1 is stimulated by NF-kB in response to inflammatory stimuli and up-regulated by TNF-alpha, IL1-beta, IGF1, GMC-SF, CD40 and phorbol ester (2-3). Bfl1 has also been shown to reduce the release of pro-apoptotic cytochrome c from mitochondria, block caspase activation and suppress apoptosis induced by p53 (4-5).
1. Lin, E. Y.; Orlofsky, A.; Berger, M. S.; Prystowsky, M. B.: J. Immun. 151: 1979-1988, 1993.
2. Lee, H. H., Dadgostar, H., Cheng, Q., Shu, J., and Cheng, G. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 9136-9141
3. Zong, W.-X., Edelstein, L. C., Chen, C., Bash, J., and Gelinas, C. (1999) Genes Dev. 13, 382-387
4. D'Sa-Eipper, C.; Subramanian, T.; Chinnadurai, G. : Cancer Res. 56: 3879-3882, 1996.
5. Arlette B. Werner, Evert de Vries, Stephen W. G. Tait, Ilja Bontjer, and Jannie Borst; J. Biol. Chem., Vol. 277, Issue 25, 22781-22788, June 21, 2002
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