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Bcl-2 Antibody

Rabbit Monoclonal Antibody

  • WB - Bcl-2 Antibody AJ1082a
    A. Western blot analysis of Jurkat cell lysate using anti-Bcl-2 RabMab (Cat. #AJ1082a), 1:1,000 dilution.
  • IHC - Bcl-2 Antibody AJ1082a
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-BCL-2 Rab Mab (Cat. #AJ1082a).
  • FC - Bcl-2 Antibody AJ1082a
    C. Flow cytometric analysis of permeabilized Jurkat cells using anti-Bcl-2 RabMAb (red)(Cat. #AJ1082a)or a rabbit IgG (negative) (green).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10415
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names E17
Calculated MW 26266 Da
Gene ID 596
Other Names Apoptosis regulator Bcl-2, BCL2
Target/Specificity A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2 was used as immunogen. The antibody does not cross-react with other Bcl-2 family members
Dilution WB~~1:1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBcl-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL2
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Cellular Location Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein
Tissue Location Expressed in a variety of tissues.
Research Areas
Citations ( 0 )


The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).


1. Hockenbery, D. et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334
2. Cleary, M.L. et al. Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell 47: 19
3. Ruvolo, P.P et al. Phosphorylation of Bcl2 and regulation of apoptosis. Leukemia 15: 515
4. Deng, X. et al. Novel Role for JNK as a Stress-activated Bcl2 Kinase. J. Biol. Chem. 276: 23681

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Cat# AJ1082a
(40 western blots)
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