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Bcl-6 Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - Bcl-6 Antibody (C-term) AJ1083a
    A. Western blot analysis on Daudi cell lysate using anti-Bcl-6 (C-term) RabMAb (Cat. #AJ1083a), dilution 1:2,000.
  • IHC - Bcl-6 Antibody (C-term) AJ1083a
    B. Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using anti-Bcl-6 (C-term) RabMAb (Cat. #AJ1083a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P41182
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP529Y
Calculated MW 78846 Da
Gene ID 604
Other Names B-cell lymphoma 6 protein, BCL-6, B-cell lymphoma 5 protein, BCL-5, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51, BCL6, BCL5, LAZ3, ZBTB27, ZNF51
Target/Specificity A synthetic peptide corresponding to residues in the C-term of human Bcl-6 was used as immunogen.
Dilution IHC~~1:50~100
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBcl-6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL6
Synonyms BCL5, LAZ3, ZBTB27, ZNF51
Function Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6- binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53- dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH- dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.
Cellular Location Nucleus
Tissue Location Expressed in germinal center T- and B-cells and in primary immature dendritic cells
Research Areas
Citations (0)

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Bcl-6 (also known as LAZ3) is a member of the POZ/zinc finger (POK) family that contains an N-terminal POZ domain and 6 C-terminal zinc finger motifs (1). Bcl-6 is a transcriptional regulator that plays an important role in lymphocyte, myocyte, male germ cells, and keratinocyte survival and or differentiation (2-3). Bcl-6 has also been found to repress a number of genes involved in B cell activation and terminal differentiation, inflammation and cell cycle regulation (4). Chromosomal aberration in Bcl-6 may be a cause of B-cell non Hodgkin lymphoma and B-cell leukemia (5).


1. Kerckaert, J.-P., Deweindt, C., Tilly, H., Quief, S., Lecocq, G., and Bastard, C. (1993) Nat. Genet. 5, 66-70
2. Dent, A. L., Shaffer, A. L., Yu, X., Allman, D., and Staudt, L. M. (1997) Science 276, 589-592
3. Kumagai, T., Miki, T., Kikuchi, M., Fukuda, T., Miyasaka, N., Kamiyama, R., and Hirosawa, S. (1999) Oncogene 18, 467-475
4. Shaeffer, A. L., Yu, X., He, Y., Boldrick, J., Chan, E. P., and Staudt, L. M. (2000) Immunity 13, 199-212
5. Baron, B. W.; Nucifora, G.; McCabe, N.; Espinosa, R., III; Le Beau, M. M.; McKeithan, T. W. : Proc. Nat. Acad. Sci. 90: 5262-5266, 1993.

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Cat# AJ1083a
(40 western blots)
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