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Bcr Antibody Phospho (pY177)

Rabbit Monoclonal Antibody

  • WB - Bcr Antibody Phospho (pY177) AJ1086a
    A. Western blot analysis on 3T3 cell lysates using anti-phospho Bcr (pY177) RabMAb (Cat. #AJ1086a), 1:2000 dilution. The membrane was either (A) untreated (B) treated with pervanadate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11274
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR576(2)Y
Calculated MW 142819 Da
Gene ID 613
Other Names Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26, BCR, BCR1, D22S11
Target/Specificity A synthetic peptide corresponding to residues surrounding tyrosine 177 of human Bcr was used as immunogen.
Dilution WB~~1:1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBcr Antibody Phospho (pY177) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCR
Synonyms BCR1, D22S11
Function GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Cellular Location Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250|UniProtKB:Q6PAJ1}
Research Areas
Citations ( 0 )


The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML) (1-2). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42 (3). Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling (4).


1. Clark, S. S., McLaughlin, J., Timmons, M., Pendergast, A. M., Ben-Neriah, Y., Dow, L. W., Crist, W., Rovera, G., Smith, S. D. and Witte, O. N. (1988). Science 239, 775-777 2. Konopka, J. B., Watanabe, S. M. and Witte, O. N. (1984). Cell 37, 1035-1042
2. Konopka, J. B., Watanabe, S. M. and Witte, O. N. (1984). Cell 37, 1035-1042
3. Boguski, M. S. and McCormick, F. (1993). Nature 366, 643-654.
4. Braselmann, S. and McCormick, F. (1995). EMBO J. 14, 4839-4848

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Cat# AJ1086a
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