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>   home   >   Products   >   Primary Antibodies   >   Microbiology   >   Beclin-1 Antibody   

Beclin-1 Antibody

Rabbit Monoclonal Antibody

     
  • WB - Beclin-1 Antibody AJ1087a
    A. Western blot analysis on A431 cell lysate using anti-Beclin-1 RabMAb (Cat. #AJ1087a), dilution 1:5000.
  • IHC - Beclin-1 Antibody AJ1087a
    B. Immunohistochemical staining of paraffin-embedded human breast tissue using anti-Beclin-1 RabMAb (Cat. #AJ1087a).
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q14457
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR1733Y
Calculated MW 51896 Da
Gene ID 8678
Other Names Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human Beclin-1 was used as an immunogen.
Dilution WB~~1:5000
IHC~~1:50
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBeclin-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BECN1
Synonyms GT197
Function Plays a central role in autophagy (PubMed:23184933). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3- C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:O88597, ECO:0000269|PubMed:19713971, ECO:0000269|PubMed:21364619}. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Endosome {ECO:0000250|UniProtKB:O88597}. Cytoplasmic vesicle, autophagosome. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) {ECO:0000250|UniProtKB:O88597, ECO:0000269|PubMed:19050071} Beclin-1-C 37 kDa: Mitochondrion {ECO:0000250|UniProtKB:O88597}
Tissue Location Ubiquitous.
Research Areas
Citations ( 0 )

Background

The Beclin-1 (BECN1) gene encodes a 60-kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity and tumorigenesis. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3).

References

1. Aita VM, et al. Genomics 59(1):59-65, 1999
2. Liang XH, et al. Nature 402(6762):672-6, 1999
3. Liang XH, et al. Cancer Res 61(8):3443-9, 2001

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Discontinued
Cat# AJ1087a
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