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Beta-Actin AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1089a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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Beta-Actin Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | P60709 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EP1123Y |
| Calculated MW | 41737 Da |
| Gene ID 60 | |
| Other Names ACTB, Actin, cytoplasmic 1, N-terminally processed, Beta-actin | |
| Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human Beta Actin (Cytoplasmic Actin) was used as an immunogen. | |
| Dilution WB~~1:1000~5000 IHC~~1:50 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Beta-Actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Beta-Actin Antibody - Protein Information | |
| Name ACTB | |
| Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells | |
| Cellular Location Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs | |
Beta-Actin Antibody - Related products
AM1021a: Beta-actin Antibody (Ascites)
Beta-Actin Antibody - Application data
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A. Western blot analysis on 1) T47D and 2) HeLa cell lysate using anti- Beta Actin (Cytoplasmic) RabMAb (Cat. #AJ1089a).
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B. Immunohistochemical analysis of paraffin-embedded human muscle using anti-Beta Actin (Cytoplasmic) RabMAb (1854-1).
Beta-Actin Antibody - Research Areas
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BACKGROUND
Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Inhibition of glycolysis with 2-deoxyglucose induced a rapid transformation of β-actin-rich pseudopodia and prevented cell motility, therefore a localized glycolytic supply of energy regulates the formation of β-actin-rich pseudopodial protrusions and thereby the motility of invasive tumor cells (2). β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error.
REFERENCES
1. Herman IM, Curr. Opin. Cell Biol. 5:48
2. Nguyen Tn, et al. Exp Cell Res. 258(1):171-83, 2000
