|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||49671 Da|
|Other Names||Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-term of human β-Tubulin was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Beta-Tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.|
|Cellular Location||Cytoplasm, cytoskeleton.|
|Tissue Location||Ubiquitously expressed with highest levels in spleen, thymus and immature brain.|
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Provided below are standard protocols that you may find useful for product applications.
Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3).
1. Wang, HW et al. Nature 435: 911-915, 2005.
2. Cleveland, DW et al. Cell 20: 95-105, 1980.
3. Tsurutani J et al. Lung Cancer 35: 11-16, 2002.
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