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Beta-Tubulin Antibody

Rabbit Monoclonal Antibody

  • WB - Beta-Tubulin Antibody AJ1097b
    A. Western blot analysis on HeLa cell lysate using anti- Beta-Tubulin RabMAb (Cat. #AJ1097b), dilution 1:20,000.
  • IHC - Beta-Tubulin Antibody AJ1097b
    B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti-Beta-Tubulin RabMAb (Cat. #AJ1097b)
  • IF - Beta-Tubulin Antibody AJ1097b
    C. Imunofluorescent staining of HeLa cells using anti- Beta-Tubulin RabMAb (Cat. #AJ1097b)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07437
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP1331Y
Calculated MW 49671 Da
Gene ID 203068
Other Names Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5
Target/Specificity A synthetic peptide corresponding to residues near the C-term of human β-Tubulin was used as immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBeta-Tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms TUBB5
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Research Areas
Citations (0)

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Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3).


1. Wang, HW et al. Nature 435: 911-915, 2005.
2. Cleveland, DW et al. Cell 20: 95-105, 1980.
3. Tsurutani J et al. Lung Cancer 35: 11-16, 2002.

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Cat# AJ1097b
(40 western blots)
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