|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||50433 Da|
|Other Names||Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB3, TUBB4|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-term of class III Beta-Tubulin was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Beta-Tubulin class III Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.|
|Cellular Location||Cytoplasm, cytoskeleton.|
|Tissue Location||Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors (3).
1. Wang, HW et al. Nature 435: 911-915, 2005.
2. Cleveland, DW et al. Cell 20: 95-105, 1980.
3. Hasegawa S et al. Clin Can Res 9: 2992-2997, 2003.
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