|Application ||WB, IHC|
|Calculated MW||22171 Da|
|Other Names||Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BCL2L11, BIM|
|Target/Specificity||A synthetic peptide corresponding to residues in the exons 2 of human Bim was used as immunogen. The antibody can recognize all isoforms of Bim. The antibody does not cross-react with other Bcl-2 family members.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Bim Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.|
|Cellular Location||Endomembrane system; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes. Isoform BimL: Mitochondrion. Isoform Bim-alpha1: Mitochondrion.|
|Tissue Location||Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney|
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Provided below are standard protocols that you may find useful for product applications.
Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members (1). Bim promotes apoptosis by binding to and inhibiting the activity of anti-apoptotic Bcl-2 family members, inducing the release of cytochrome c from mitochondria (2). Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis (3,4).
1. O'Connor, L., et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 17: 384?395 (1998).
2. Hsu, S.Y., et al. BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 12: 1432
3. Puthalakath, H., et al. The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex. Mol. Cell 3: 287
4. Strasser, A., et al. The role of Bim, a proapoptotic BH3-only member of the Bcl-2 family in cell-death control. Ann N Y Acad Sci. 917: 541
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