|Application ||WB, IHC|
|Other Names||BLNK, BASH, SLP65, B-cell linker protein, Cytoplasmic adapter protein;B-cell adapter containing a SH2 domain protein;B-cell adapter containing a Src homology 2 domain protein;Src homology 2 domain-containing leukocyte protein of 65 kDa|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-term of human BLNK was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BLNK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
B-cell linker protein (BLNK, also known as SLP65, BASH, and BCA) is a B cell adaptor that plays an important role in B cell development and in BCR-signal transduction. Since BLNK does not encode any intrinsic enzymatic activity, its function is to serve as a scaffold for assembling macromolecular complexes which includes enzymes (PLC gamma, Vav and Btk) and additional linker proteins (Grb2 and Nck) (1-3) . In addition, BLNK binds Btk and is required for activation of the transcription factor NF-κB. Tyrosine phosphorylation of BLNK by Syk provides specific docking sites for these SH2-containing effector proteins which in turn permits phosphorylation and/or activation of their respective signaling pathways.
1. Fu C., Turck,C., Kurosaki,T. and Chan,A. (1998) Immunity, 9, 93
2. Jumaa H., Mitterer,M., Reth,M. and Nielsen,P.J. (2001) Eur. J. Immunol., 31, 2164
3. Hashimoto S. et al. (1999). Blood, 94, 2357
4. Christopher W. Chiu, Mark Dalton, Masamichi Ishiai,Tomohiro Kurosaki,and Andrew C. Chan; EMBO J. 2002 December 2; 21(23): 6461
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