|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||78764 Da|
|Other Names||Myb-related protein B, B-Myb, Myb-like protein 2, MYBL2, BMYB|
|Target/Specificity||A phospho specific peptide corresponding to residues surrounding threonine 487 of human B-Myb was used as an immunogen. This antibody detects B-Myb phosphorlyated on threonine 487.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||B-Myb Antibody Phospho (pT487) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.|
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Provided below are standard protocols that you may find useful for product applications.
Expression of the B-Myb transcription factor is upregulated during late G1 phase of the cell cycle by an E2F-dependent transcriptional mechanism. B-Myb is specifically phosphorylated during S phase, suggesting that a cyclin-dependent kinase (Cdk) regulates its activity. Consistent with this notion, the S phase-specific cyclin A/Cdk2 was found previously to enhance B-Myb transactivation activity in cotransfected cells. There is evidence that B-Myb is a direct physiological target for cyclin A/Cdk2. Data indicate that phosphorylation by cyclin A/Cdk2 is directly involved in enhancing B-Myb transactivation activity and that levels of endogenous cyclin A/Cdk2 activity may contribute to cell line-specific B-Myb function (1). Nuclear entry of B-Myb is dependent on multiple nuclear localization signals (NLS's). Mutagenesis of the putative NLS's of B-Myb has identified two separate NLS's, NLS1 and NLS2. Each of the two NLS's is essential for efficient nuclear targeting (2).
1. Saville MK, et al. Oncogene 17(21):2679-89, 1998
2. Takemoto Y, et al. FEBS Lett 350(1):55-60, 1994
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