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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   B-Myb Antibody Phospho (pT487)   

B-Myb Antibody Phospho (pT487)

Rabbit Monoclonal Antibody

  • WB - B-Myb Antibody Phospho (pT487) AJ1103a
    A. Western blot analysis on Raji cell lysates using anti-Phospho-B-Myb (pT487) RabMAb (Cat. #AJ1103a), 1:1000 dilution. Cells were either (A) untreated (B) treated with TPA + IGF
  • IHC - B-Myb Antibody Phospho (pT487) AJ1103a
    B. Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using anti-B-Myb RabMAb (Cat. #AJ1103a)
  • IF - B-Myb Antibody Phospho (pT487) AJ1103a
    C. Immunofluorescent staining of HeLa cells using Phospho-anti-B-Myb RabMAb(Cat. #AJ1103a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10244
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR2204Y
Calculated MW 78764 Da
Gene ID 4605
Other Names Myb-related protein B, B-Myb, Myb-like protein 2, MYBL2, BMYB
Target/Specificity A phospho specific peptide corresponding to residues surrounding threonine 487 of human B-Myb was used as an immunogen. This antibody detects B-Myb phosphorlyated on threonine 487.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB-Myb Antibody Phospho (pT487) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYBL2
Synonyms BMYB
Function Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
Cellular Location Nucleus.
Research Areas
Citations (0)

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Expression of the B-Myb transcription factor is upregulated during late G1 phase of the cell cycle by an E2F-dependent transcriptional mechanism. B-Myb is specifically phosphorylated during S phase, suggesting that a cyclin-dependent kinase (Cdk) regulates its activity. Consistent with this notion, the S phase-specific cyclin A/Cdk2 was found previously to enhance B-Myb transactivation activity in cotransfected cells. There is evidence that B-Myb is a direct physiological target for cyclin A/Cdk2. Data indicate that phosphorylation by cyclin A/Cdk2 is directly involved in enhancing B-Myb transactivation activity and that levels of endogenous cyclin A/Cdk2 activity may contribute to cell line-specific B-Myb function (1). Nuclear entry of B-Myb is dependent on multiple nuclear localization signals (NLS's). Mutagenesis of the putative NLS's of B-Myb has identified two separate NLS's, NLS1 and NLS2. Each of the two NLS's is essential for efficient nuclear targeting (2).


1. Saville MK, et al. Oncogene 17(21):2679-89, 1998
2. Takemoto Y, et al. FEBS Lett 350(1):55-60, 1994

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Cat# AJ1103a
(40 western blots)
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