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BNP Antibody

Rabbit Monoclonal Antibody

  • WB - BNP Antibody AJ1105a
    Western blot analysis on (A) human plasma and (B) fetal heart lysates using anti-BNP RabMAb (Cat. #AJ1105a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16860
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR3735
Calculated MW 14726 Da
Gene ID 4879
Other Names Natriuretic peptides B, Gamma-brain natriuretic peptide, Brain natriuretic peptide 32, BNP(1-32), BNP-32, BNP(1-30), BNP(1-29), BNP(1-28), BNP(2-31), BNP(3-32), BNP(3-30), BNP(3-29), BNP(4-32), BNP(4-31), BNP(4-30), BNP(4-29), BNP(4-27), BNP(5-32), BNP(5-31), BNP(5-29), NPPB
Target/Specificity A synthetic peptide corresponding to residues in human BNP was used as an immunogen.
Dilution WB~~1:2000~20000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBNP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
Cellular Location Secreted.
Tissue Location Brain and also in atria, but at much lower levels than ANP
Research Areas
Citations (0)

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B-type natriuretic peptide (BNP) is a cardiac neurohormone specifically secreted from the ventricles in response to volume expansion and pressure overload (1). The actions of BNP include natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. It is also found to help restore the body's salt and water balance and improve heart function (2). BNP is co-secreted along with a biologically inactive N-terminal fragment (NT-proBNP). Circulating BNP and NT-proBNP provide important information on cardiac dysfunction, hypervolemia, and risk in patients with severe impairment of kidney function (3).


1. Maisel AS, et al. N Engl J Med. 347(3):161-7, 2002 2. The UniProt Consortium. The Universal Protein Resource (UniPrto), Nucleic Acids Res. 36:D190-D195 (2008) 3. Khalifeh N., et al. J Investig Med. 57(1):33-9, 2009

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Cat# AJ1105a
(40 western blots)
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