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B-Raf Antibody Phospho (pT401)

Rabbit Monoclonal Antibody

  • WB - B-Raf Antibody Phospho (pT401) AJ1106b
    A. Western blot analysis on PC-12 cell lysates using anti-Phospho-B-Raf (p401) RabMAb (Cat. #AJ1106b), 1:5000 dilution. Cells were either (A) untreated (B) treated with TPA.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15056
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR2208Y
Calculated MW 84437 Da
Gene ID 673
Other Names Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
Target/Specificity A phospho specific peptide corresponding to residues surrounding threonine 401 of human B-Raf was used as an immunogen. This antibody detects B-Raf phosphorylated on threonine 401.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB-Raf Antibody Phospho (pT401) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BRAF1, RAFB1
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Location Brain and testis.
Research Areas
Citations (0)

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B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.


1. Hagemann C., Rapp U. R. Cell Res., 253: 34-46, 1999
2. Heidecker et al. 1990 Mol. Cell. Biol. 10:2503-2512.
3. Jaiswal, R. K.,et al. (1994) Mol. Cell. Biol. 14, 6944-6953
4. Brose et al. Cancer Res., 62: 6997-7000, 2002

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Cat# AJ1106b
(40 western blots)
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