|Reactivity||Human, Mouse, Rat|
|Calculated MW||84437 Da|
|Other Names||Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1|
|Target/Specificity||A phospho specific peptide corresponding to residues surrounding threonine 401 of human B-Raf was used as an immunogen. This antibody detects B-Raf phosphorylated on threonine 401.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||B-Raf Antibody Phospho (pT401) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.|
|Cellular Location||Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.|
|Tissue Location||Brain and testis.|
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Provided below are standard protocols that you may find useful for product applications.
B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.
1. Hagemann C., Rapp U. R. Cell Res., 253: 34-46, 1999
2. Heidecker et al. 1990 Mol. Cell. Biol. 10:2503-2512.
3. Jaiswal, R. K.,et al. (1994) Mol. Cell. Biol. 14, 6944-6953
4. Brose et al. Cancer Res., 62: 6997-7000, 2002
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