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BRG1 Antibody

Rabbit Monoclonal Antibody

  • WB - BRG1 Antibody AJ1107a
    Western blot analysis on A) K562 and B) Molt-4 cell lysates using anti-BRG1 RabMAb (Cat. #AJ1107a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51532
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EPR3913
Calculated MW 184646 Da
Gene ID 6597
Other Names Transcription activator BRG1, 364-, ATP-dependent helicase SMARCA4, BRG1-associated factor 190A, BAF190A, Mitotic growth and transcription activator, Protein BRG-1, Protein brahma homolog 1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4, SMARCA4, BAF190A, BRG1, SNF2B, SNF2L4
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human BRG1 protein was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBRG1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BAF190A, BRG1, SNF2B, SNF2L4
Function Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self- renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00549, ECO:0000269|PubMed:20418909}
Tissue Location Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level)
Research Areas
Citations (0)

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BRG1 protein (brahma-related gene 1) is a member of SWI/SNF family which mediates ATP-dependent chromatin remodeling processes. BRG is a central catalytic ATPase of the SWI/SNF chromatin-remodeling complex, where it uses the energy derived from ATP-hydrolysis to disrupt the chromatin architecture of target promoters. Considered a major co-regulator of transcription, BRG1 has been implicated in the activation and repression of gene expression through the modulation of chromatin in various tissues and physiological conditions. BRG1 appears to be a critical modulator of transcriptional regulation in cellular processes such as transcriptional regulation, replication, DNA repair and recombination (1, 2).


1. Reisman D et al. Oncogene. 28(14):1653-682, 2009. 2. KW Trotter and TK Archer, Nucl Recept Signal. 2008

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Cat# AJ1107a
(40 western blots)
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