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CAD Antibody (N-term)

Rabbit Monoclonal Antibody

  • WB - CAD Antibody (N-term) AJ1114b
    A. Western blot analysis on 293 cell lysate using anti-CAD (N-term) RabMAb (Cat. #AJ1114b), dilution 1:1,000.
  • IF - CAD Antibody (N-term) AJ1114b
    B. Immunofluorescent staining of HeLa cells using anti-CAD (N-term) RabMAb (Cat. #AJ1114b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P27708
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP710Y
Calculated MW 242984 Da
Gene ID 790
Other Names CAD protein, Glutamine-dependent carbamoyl-phosphate synthase, Aspartate carbamoyltransferase, Dihydroorotase, CAD
Target/Specificity A synthetic peptide corresponding to residues in the N-terminus of human CAD was used as immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCAD Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CAD
Function This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
Cellular Location Cytoplasm. Nucleus. Note=Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth
Research Areas
Citations (0)

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Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis (1). This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway (2). Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus (3). While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD (4-5).


1. Chen, et al. Hum. Genet. 82: 40-44, 1989.
2. Jones, M.E. (1980) Annu. Rev. Biochem., 49, 253
3. Sigoillot, F. D., Berkowski, A. J., Sigoillot, S. M., Kotsis, D. H., and Guy, H. I. (2003) J. Biol. Chem. 278,3403
4. Evans, D. R., and Guy, H. I. (2004) J. Biol. Chem. 279,33035
5. Graves et al. (2000) Nature, 403, 328

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Cat# AJ1114b
(40 western blots)
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