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CAD Antibody (N-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1114b | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
CAD Antibody (N-term) - Product info | |
| Application | WB, IF
|
| Primary Accession | P27708 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EP710Y |
| Calculated MW | 242984 Da |
| Gene ID 790 | |
| Other Names CAD, Dihydroorotase | |
| Target/Specificity A synthetic peptide corresponding to residues in the N-terminus of human CAD was used as immunogen. | |
| Dilution WB~~1:1000~10000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions CAD Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
CAD Antibody (N-term) - Protein Information | |
| Name CAD | |
| Function This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase) | |
| Cellular Location Cytoplasm. | |
CAD Antibody (N-term) - Related products
CAD Antibody (N-term) - Application data
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A. Western blot analysis on 293 cell lysate using anti-CAD (N-term) RabMAb (Cat. #AJ1114b), dilution 1:1,000.
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B. Immunofluorescent staining of HeLa cells using anti-CAD (N-term) RabMAb (Cat. #AJ1114b).
CAD Antibody (N-term) - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis (1). This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway (2). Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus (3). While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD (4-5).
REFERENCES
1. Chen, et al. Hum. Genet. 82: 40-44, 1989.
2. Jones, M.E. (1980) Annu. Rev. Biochem., 49, 253
3. Sigoillot, F. D., Berkowski, A. J., Sigoillot, S. M., Kotsis, D. H., and Guy, H. I. (2003) J. Biol. Chem. 278,3403
4. Evans, D. R., and Guy, H. I. (2004) J. Biol. Chem. 279,33035
5. Graves et al. (2000) Nature, 403, 328
