|Application ||WB, IHC|
|Calculated MW||51926 Da|
|Other Names||Calcium/calmodulin-dependent protein kinase type IV, CaMK IV, CaM kinase-GR, CAMK4, CAMK, CAMK-GR, CAMKIV|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human CaMKIV was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Calcium/Calmodulin Kinase IV (CaMKIV) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CAMK, CAMK-GR, CAMKIV|
|Function||Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.|
|Cellular Location||Cytoplasm. Nucleus. Note=Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity).|
|Tissue Location||Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue|
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Ca(2+)/calmodulin-dependent protein kinase IV (CaMKIV) is a multifunctional protein kinase expressed abundantly in the central nervous system. Because changes in intracellular Ca(2+) concentrations affect progression through the mitotic cell cycle, enhanced expression of CaMKIV has been reported in small cell lung carcinoma and hepatocellular carcinoma (1). Multifunctional Ca2+/calmodulin kinase IV is enriched in neurons and T lymphocytes. The kinase is absent from primary human B lymphocytes but is expressed in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cell lines, suggesting that expression of the kinase can be upregulated by an EBV gene products. In view of the key role played by LMP1 in B-lymphocyte immortalization by EBV, findings implicate Ca2+/calmodulin kinase IV as a potential mediator of B-lymphocyte growth transformation (2). Ca2+/calmodulin kinase IV can suppress the MT-regulating activity of Op18 by phosphorylation on Ser-16. These results show that Op18 phosphorylation by Ca2+/calmodulin kinase IV may couple alterations of MT dynamics in response to external signals that involve Ca2+ (3).
1. Takai N, et al. Cancer Lett, 183(2) 185-93, 2002.
2. Mosialos G, et al. J Virol 68(3): 1697-705, 1994
3. Melander Gradin H, et al. Mol Cell Biol 17(6): 3459-67, 1997.
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