|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||93231 Da|
|Other Names||Caldesmon, CDM, CALD1, CAD, CDM|
|Target/Specificity||A synthetic phospho-peptide corresponding to residues surrounding Ser789 of human Caldesmon was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Caldesmon Antibody (CaM) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).|
|Cellular Location||Cytoplasm, cytoskeleton. Cytoplasm, myofibril. Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle)|
|Tissue Location||High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart|
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Provided below are standard protocols that you may find useful for product applications.
Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).
1. Humphrey, M.B., et al. Cloning of cDNAs encoding human caldesmons. Gene 112: 197
2. Bryan, J., et al. Cloning and expression of a smooth muscle caldesmon. J Biol Chem. 264: 13873
3. Bryan, J. Caldesmon: fragments, sequence, and domain mapping. Ann N Y Acad Sci. 599: 100
4. Fraser, I.D., et al. The inhibitory complex of smooth muscle caldesmon with actin and tropomyosin involves three interacting segments of the C-terminal domain 4. Biochemistry 36: 5483
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