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Caldesmon Antibody Phospho (pT730)

Rabbit Monoclonal Antibody

  • WB - Caldesmon Antibody Phospho (pT730) AJ1117d
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-Caldesmon (pT730) RabMAb (Cat. #AJ1117d), 1:10,000 dilution. Cells were either (A) untreated (B) treated with Calyculin A.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q05682
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR2210
Calculated MW 93231 Da
Gene ID 800
Other Names Caldesmon, CDM, CALD1, CAD, CDM
Target/Specificity A phospho-specific peptide corresponding to residues surrounding Threonine 730 of human Caldesmon was used as an immunogen. The antibody only detects Caldesmon phosphorylated on Threonine 730.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCaldesmon Antibody Phospho (pT730) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CALD1
Synonyms CAD, CDM
Function Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, myofibril. Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle)
Tissue Location High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart
Research Areas
Citations (0)

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Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).


1. Humphrey MB, et al. Gene 112:197
2. Bryan J, et al. J Biol Chem 264:13873
3. Bryan J, et al. Ann N Y Acad Sci 599:100
4. Fraser ID, et al. Biochem 36:5483

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Cat# AJ1117d
(40 western blots)
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