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Calpain-1 Antibody

Rabbit Monoclonal Antibody

  • WB - Calpain-1 Antibody AJ1121a
    A. Western blot analysis on (A) T47D, (B) fetal brain, (C) fetal spleen and (D) 293T cell lysates using anti-Calpain 1 (small subunit) RabMAb (Cat. #AJ1121a), dilution 1:2000.
  • IHC - Calpain-1 Antibody AJ1121a
    B. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 1 (small subunit) RabMAb (Cat. #AJ1121a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04632
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3324
Calculated MW 28316 Da
Gene ID 826
Other Names Calpain small subunit 1, CSS1, Calcium-activated neutral proteinase small subunit, CANP small subunit, Calcium-dependent protease small subunit, CDPS, Calcium-dependent protease small subunit 1, Calpain regulatory subunit, CAPNS1, CAPN4, CAPNS
Target/Specificity A synthetic peptide corresponding to residues in human Calpain 1 (small subunit) was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCalpain-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms CAPN4, CAPNS
Function Regulatory subunit of the calcium-regulated non- lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Cellular Location Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon calcium binding.
Research Areas
Citations (0)

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Calcium-dependent cysteine proteinases, or calpains, are widely distributed in mammalian cells. There are 2 distinct molecular forms, calpains 1 and 2, which differ in the quantity of calcium required. Both calpains 1 and 2 are heterodimeric; each is composed of 1 heavy (about 80 kD), CAPN1 and CAPN2, respectively, and 1 shared light (about 30 kD) subunit, CAPNS1. The heavy subunit has a catalytic function, and the light subunit is regulatory (1).


1. Online Mendelian Inheritance in Man (OMIM), John Hopkins University, Calpain, small subunit 1;CAPNS1 (114170)

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Cat# AJ1121a
(40 western blots)
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