|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||33170 Da|
|Other Names||Calponin-1, Basic calponin, Calponin H1, smooth muscle, CNN1|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-term of human Calponin was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Calponin-1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).|
|Tissue Location||Smooth muscle, and tissues containing significant amounts of smooth muscle|
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Provided below are standard protocols that you may find useful for product applications.
Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).
1. Strasser P, et al. FEBS Lett. 330(1):13-8, 1993
2. Ferjani I, et al. FEBS Lett. 580(20):4801-6, 2006
3. Prasad AR, et al. Arch Pathol Lab Med. 123(9): 801-6, 1999
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