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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Calreticulin Antibody   

Calreticulin Antibody

Rabbit Monoclonal Antibody

     
  • WB - Calreticulin Antibody AJ1124a
    Fig 1. Western blot analysis on (A) SH-SY5Y, (B) HL-60, (C) HepG2, (D) HeLa, (E) Fetal kidney and (F) Fetal brain lysates using anti-Calreticulin RabMAb (Cat. #AJ1124a).
  • IF - Calreticulin Antibody AJ1124a
    Fig 3. Immunofluorescent staining of HeLa cells using anti-Calreticulin RabMAb (Cat. #AJ1124a).
  • IHC - Calreticulin Antibody AJ1124a
    Fig 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calreticulin RabMAb (Cat. #AJ1124a).
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession P27797
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3924
Calculated MW 48142 Da
Gene ID 811
Other Names Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, grp60, CALR, CRTC
Target/Specificity A synthetic peptide corresponding to residues in human Calreticulin was used as an immunogen.
Dilution WB~~1:1000~10000
IHC~~1:250~500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCalreticulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CALR
Synonyms CRTC
Function Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).
Cellular Location Endoplasmic reticulum lumen Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Sarcoplasmic reticulum lumen {ECO:0000250|UniProtKB:P28491}. Note=Also found in cell surface (T cells), cytosol and extracellular matrix (PubMed:10358038). Associated with the lytic granules in the cytolytic T-lymphocytes.
Research Areas
Citations ( 0 )

Background

Calreticulin is a molecular Ca+ binding chaperone promoting folding, oligomeric assembly, and quality control in ER. Calreticulin is a highly versatile lectin-like chaperone, and it participates during the synthesis of a variety of molecules, including ion channels, surface receptors, integrins and transporters (1). Calreticulin indeed provides up to 45% of the Ca2+-buffering capacity for a pool of the IP3-sensitive Ca2+ inside the ER (2). Calreticulin also functions in cell adhesion, integrin-dependent Ca2+ signaling and steroid-sensitive gene [removed]1).

References

1. Michalak et al. Biochem. J. 344: 281-292, 1999. 2. Hayashi et al. Trends Cell Biol. 19(2) : 81-88, 2009.

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Discontinued
Cat# AJ1124a
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