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CaMKI AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1126b | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
CaMKI Antibody - Product info | |
| Application | WB, IHC
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| Primary Accession | Q14012 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EPR2217Y |
| Calculated MW | 41337 Da |
| Gene ID 8536 | |
| Other Names CAMK1, Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I alpha | |
| Target/Specificity A specific peptide corresponding to residues on the N-terminus of human CaMKI was used as an immunogen. | |
| Dilution WB~~1:5000~10000 IHC~~1:100 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions CaMKI Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
CaMKI Antibody - Protein Information | |
| Name CAMK1 | |
| Function Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF] Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser- 694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser- 498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen- stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I | |
| Cellular Location Cytoplasm (By similarity). Nucleus (By similarity). Note=Predominantly cytoplasmic (By similarity) | |
| Tissue Location Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex | |
CaMKI Antibody - Related products
AP7205a: CAMK1 Antibody (C-term)
RI10757: CAMK1 predesign siRNA
LY10293a: CAMK1 Over-expression Lysate
CaMKI Antibody - Application data
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A. Western blot analysis on (A) SH-SY5Y (B) human brain (C) mouse brain (D) rat brain lysate using anti-CaMKI RabMAb (Cat. #AJ1126b), dilution 1:5000.
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B. Immunohistochemical analysis of paraffin-embedded human kidney using anti-CaMK1 RabMAb (Cat. #AJ1126b).
CaMKI Antibody - Research Areas
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BACKGROUND
Ca(+2)/calmodulin-dependent protein kinases (CaMKs) are activated upon binding of Ca(+2)/calmodulin. To gain maximal activity, CaMK I and CaMK IV can be further phosphorylated by an upstream kinase, CaMK kinase (CaMKK) (1). CaMKI is a Ca2+/calmodulin-dependent protein kinase that is widely expressed in eukaryotic cells and tissues but for which few, if any, physiological substrates are known (2). CaMKI has been shown to comprise a family of four structurally related isoforms (alpha, beta, gamma, and delta) encoded by separate genes with abundant expression in mature brain. In mouse brain, the four isoforms of CaMKI were found to exhibit distinct spatiotemporal expression during neuronal development. CaMKI may also have possible functional involvement of in the basal dendritic growth of hippocampal neurons (3)
REFERENCES
1. Hsu LS, et al. J Biol Chem 276(33):31113-23, 2001
2. Qin H, et al. J Biol Chem 278(49):48570-9, 2003
3. Kamata A, et al. Neurosci Res 57(1): 86-97, 2007
