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Caspase-10 Antibody (Pro)

Rabbit Monoclonal Antibody

  • WB - Caspase-10 Antibody (Pro) AJ1129a
    A. Western blot analysis of HeLa cell lysate using anti-Pro-Caspase-10 RabMab (Cat. #AJ1129a), 1,000 dilution.
  • IHC - Caspase-10 Antibody (Pro) AJ1129a
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Caspase 10 RabMab (Cat. #AJ1129a).
  • IF - Caspase-10 Antibody (Pro) AJ1129a
    C. Caspase 10 expression in apoptotic HeLa cells detected by Immunofluorescent staining using anti-Pro-Caspase 10 RAbMab (Cat. #AJ1129a).
  • FC - Caspase-10 Antibody (Pro) AJ1129a
    D. Flow cytometric analysis of permeabilized HeLa cells using anti-Pro-Caspase-10 RabMAb (red) (Cat. #AJ1129a)or a rabbit IgG (negative) (green).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92851
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names E35
Calculated MW 58951 Da
Gene ID 843
Other Names Caspase-10, CASP-10, Apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1B-converting enzyme 2, FLICE2, ICE-like apoptotic protease 4, Caspase-10 subunit p23/17, Caspase-10 subunit p12, CASP10, MCH4
Target/Specificity A synthetic peptide corresponding to N-terminal residues of human Caspase-10 subunit p23/17 was used as immunogen. The antibody does not cross-react with other Caspase family members.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCaspase-10 Antibody (Pro) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CASP10
Synonyms MCH4
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.
Tissue Location Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon
Research Areas
Citations (0)

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Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-10 (MCH4, FLICE2) is one of the initiator caspases (1,2), and is localized to various tissues. Pro-caspase-10 has two death effector domains (DEDs) that bind FADD and recruits both TNFR1 and CD95 to form complexes with these receptors and induce apoptosis (1,2). This death domain complex cleaves pro-caspase-10 into a large active fragment and a small fragment. Cleaved caspase-10 leads to the processing of caspase-3 and caspase-7, initiating a caspase cascade and subsequent apoptosis (3,4). It has been shown that the Caspase-10 protein is not produced in neither mouse nor rat (5).


1. Vincenz, C. and Dixit, V.M. Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling. J. Biol. Chem. 272: 6578
2. Fernandes-Alnemri, T. et al. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc. Natl. Acad. Sci., USA 93: 7464
3. Srinivasula, S.M. et al. Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases. Proc. Natl. Acad. Sci., USA 93: 14486
4. Wang, J. et al. Inherited human Caspase 10 mutations underlie defective lymphocyte and dendritic cell apoptosis in autoimmune lymphoproliferative syndrome type II. Cell 98: 47
5. Janicke RU, Science 312(5782): 1874, 2006.

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Cat# AJ1129a
(40 western blots)
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