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Caspase-3 Antibody (Active)

Rabbit Monoclonal Antibody

  • WB - Caspase-3 Antibody (Active) AJ1131b
    A. Western blot analysis on Jurkat cell lysates using anti-Caspase-3 RabMAb (Cat. #AJ1131b). Cells were either (A) untreated or (B) treated with Camptothecin.
  • IF - Caspase-3 Antibody (Active) AJ1131b
    B. Immunofluorescent staining of untreated HeLa cells using anti-Caspase-3 RabMAb (Cat. #AJ1131b).
  • IF - Caspase-3 Antibody (Active) AJ1131b
    C. Immunofluorescent staining of HeLa cells treated with staurosporine using anti-Caspase-3 RabMAb (Cat. #AJ1131b) showing activation of Caspase-3 in these cells.
  • IHC - Caspase-3 Antibody (Active) AJ1131b
    D. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-Caspase-3 RabMAb (Cat. #AJ1131b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P42574
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names E83-77
Calculated MW 31608 Da
Gene ID 836
Other Names Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, CASP3, CPP32
Target/Specificity A synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit) was used as immunogen. This antibody only detects the active form of caspase-3 (p17 subunit).
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCaspase-3 Antibody (Active) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CASP3
Synonyms CPP32
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system
Research Areas
Citations ( 0 )


Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).


1. Fernandes-Alnemri, T. et al. J. Biol. Chem. 269:30761-4, 1994
2. Tewari, M. et al. Cell 81: 801-9, 1995
3. Nicholson, D.W. et al. Nature 376: 37-43, 1995

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Cat# AJ1131b
(40 western blots)
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