|Application ||IHC, WB|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||59756 Da|
|Other Names||Catalase, CAT|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human Catalase was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Catalase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.|
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Provided below are standard protocols that you may find useful for product applications.
Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1. 5 A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erythrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflammation) (2).
1. Putnam CD, et al. J Mol Biol 296(1):295-309, 2000
2. Jin LH, et al. Free Radic Biol Med 31(11):1509-19, 2001
3. Agar NS, et al. J Clin Invest 77(1):319-21, 1986
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