|Calculated MW||99633 Da|
|Other Names||E3 ubiquitin-protein ligase CBL, 632-, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, Signal transduction protein CBL, CBL, CBL2, RNF55|
|Target/Specificity||A phospho specific peptide corresponding to residues surrounding tyrosine 674 of human Cbl were used as an immunogen. This antibody detects Cbl phosphorylated on tyrosine 674.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Cbl Antibody Phospho (pY674) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.|
|Cellular Location||Cytoplasm. Cell membrane. Note=Colocalizes with FGFR2 in lipid rafts at the cell membrane|
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Provided below are standard protocols that you may find useful for product applications.
c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4). It has been demonstrated that certain tyrosines are the major phosphorylation sites of c-Cbl in T cells in response to pervanadate treatment, as well as in response to TcR/CD3 ligation. Cbl also enhances migration of v-Abl-transformed NIH 3T3 fibroblasts. This effect of Cbl depends on its tyrosine phosphorylation, which is required for binding of PI-3' kinase to c-Cbl (5)
1. Langdon, W. Y., et al. Proc. Natl. Acad. Sci. USA 86:1168-72 (1989)
2. Blake, T. J., et al. EMBO J. 12: 2017-26 (1993)
3. Joazeiro, C. A., et al. Science 286: 309-12 (1999)
4. Feshchenko EA, et al. J Biol Chem, 272(14):8323-31, 1998
5. Teckchandani AM, et al. Exp Cell Res 307(1): 247-58,2005
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