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Cbl Antibody Phospho (pY700)

Rabbit Monoclonal Antibody

  • WB - Cbl Antibody Phospho (pY700) AJ1142d
    A. Western blot analysis on Jurkat cell lysate using anti-Phospho-Cbl (pY700) RabMAb (Cat. #AJ1142d), 1:10,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P22681
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP2225Y
Calculated MW 99633 Da
Gene ID 867
Other Names E3 ubiquitin-protein ligase CBL, 632-, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, Signal transduction protein CBL, CBL, CBL2, RNF55
Target/Specificity A phospho specific peptide corresponding to residues surrounding tyrosine 700 were used as an immunogen. This antibody detects Cbl phosphorylated on tyrosine 700.
Dilution WB~~1:5000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCbl Antibody Phospho (pY700) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBL
Synonyms CBL2, RNF55
Function Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
Cellular Location Cytoplasm. Cell membrane. Note=Colocalizes with FGFR2 in lipid rafts at the cell membrane
Research Areas
Citations (0)

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c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4). It has been demonstrated that tyrosines 700, 731, and 774 are the major tyrosine phosphorylation sites of c-Cbl in T cells in response to pervanadate treatment, as well as in response to TcR/CD3 ligation (5).


1. Langdon, W. Y., et al. Proc. Natl. Acad. Sci. USA 86: 1168-72 (1989).
2. Blake, T. J., et al. EMBO J. 12: 2017-26 (1993).
3. Joazeiro, C. A., et al. Science 286: 309-12 (1999).
4. Feshchenko EA, et al. J Biol Chem, 272(14):8323-31, 1998

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Cat# AJ1142d
(40 western blots)
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