|Application ||WB, IF|
|Calculated MW||41731 Da|
|Other Names||Choline-phosphate cytidylyltransferase A, CCT-alpha, CTP:phosphocholine cytidylyltransferase A, CCT A, CT A, Phosphorylcholine transferase A, PCYT1A, CTPCT, PCYT1|
|Target/Specificity||A synthetic peptide corresponding to residues in human CCT-alpha was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CCT-alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Controls phosphatidylcholine synthesis.|
|Cellular Location||Cytoplasm, cytosol. Membrane; Peripheral membrane protein. Note=It can interconvert between an inactive cytosolic form and an active membrane-bound form.|
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Provided below are standard protocols that you may find useful for product applications.
CTP:phosphocholine cytidylyltransferase (CCT) is the key regulator enzyme in the synthesis of phosphatidylcholine (PC), the most abundant phospholipid in eukaryotic cell membranes. CCT catalyzes the transfer of a cytidylyl group from CTP to phosphocholine to form CDP-choline (1). A steady, albeit low, production of PC is necessary to maintain suitable PC content for lipid homeostasis. However, elevated activity of CCT is required during cell division when increased PC production is needed for membrane biogenesis (2). To date, three CCT isoforms have been identified: CCT-alpha, CCT-beta 1, and CCT-beta 2 (3). CCT-alpha is localized to the nucleus and nuclear envelope in many cell types. An exception is lung-derived cell, where CCT-alpha is localized primarily in cytoplasmic and ER compartments. CCT-alpha is involved in important nuclear functions such as coordinating PC synthesis with cell-cycle regulation and regulating proliferation of the nucleoplasmic reticulum, an intranuclear membrane network implicated in calcium signaling and nuclear-cytoplasmic transport (4).
1. Lee, J., et al. J Biol Chem. 2009 2. Ridsdale et al. J. Biol. Chem. 276 (52) : 49148-49155, 2001. 3. Lykidis et al. J. Biol. Chem. 273 : 14022-14029. 4. Lagace, T., et al. Biochem. J. 392: 449-456, 2005
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