|Calculated MW||85514 Da|
|Other Names||Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, Enkephalinase, Neutral endopeptidase 2411, NEP, Neutral endopeptidase, Skin fibroblast elastase, SFE, CD10, MME, EPN|
|Target/Specificity||A synthetic peptide corresponding to residues on human CD10 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).|
|Cellular Location||Cell membrane; Single-pass type II membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
The cluster designation (CD) antigens are cell surface molecules first defined on human leukocytes and later found to be expressed by a variety of human cell types in both normal and pathologic states. The common acute lymphoblastic leukemia antigen (CALLA/CD10) is a single-pass type II transmembrane metallo-endopeptidase that cleaves and inactivates a variety of peptide growth factors important for signal transduction including the enkephalins, bombesin, and substance P (1). It is expressed in a wide variety of epithelial and non-epithelial tissues, but its most significant application is in the diagnosis and classification of certain types of malignant lymphoma and leukemia. CD10 is expressed in a high percentage of cases of acute lymphoblastic leukemia, follicular lymphoma, Burkitt's lymphoma, and some hematopoietic tumors (2). It is also known to be a marker of endometrial stromal cells, and was reported to be expressed in cells immediately surrounding the neoplastic glands invading the myometrium (3).
1. Dall'Era M A, et al. BMC Urology 7:3, 2007
2. McIntosh, G., et al. American Journal of Pathology 154:77-82, 1999
3. Ohishi Y, et al. Hum Pathol. 39(10):1446-53, 2008
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