|Reactivity||Human, Mouse, Rat|
|Calculated MW||85514 Da|
|Other Names||Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, Enkephalinase, Neutral endopeptidase 2411, NEP, Neutral endopeptidase, Skin fibroblast elastase, SFE, CD10, MME, EPN|
|Target/Specificity||A synthetic peptide corresponding to residues on the N-terminus of human CD10 was used as an immogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).|
|Cellular Location||Cell membrane; Single-pass type II membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
CD10 is zinc-dependant metalloprotease enzyme that plays a role in the destruction of opiod peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (1). CD10 is a cell-surface glycoprotein expressed on most acute lymphoblastic leukemias and certain other immature lymphoid malignancies and on normal lymphoid progenitors. The latter are either uncommitted to B- or T-cell lineage or committed to only the earliest stages of B- or T-lymphocyte maturation. CD10 is not restricted to leukemic cells, however, and is found on a variety of normal tissues. CD10 is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium (2). The proteolysis of beta-amyloid (Abeta) requires CD10, an enzyme that has been shown as reduced in Alzheimer's disease (AD). The defect of CD10 appears to correlate with Abeta deposition but not with degeneration and dementia (3).
1. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195 (2008)
2. Shipp MA, et al. Natl Acad Sci 85(13):4819-23, 1988
3. Russo R, et al. FEBS Lett 579(27) :6027-30, 2005
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