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CD146 Antibody

Rabbit Monoclonal Antibody

  • WB - CD146 Antibody AJ1152a
    A. Western blot analysis on (A) A375, (B) fetal artery and (C) HUVEC cell lysates using anti-CD146 RabMAb (Cat. #AJ1152a), dilution 1:50,000.
  • IHC - CD146 Antibody AJ1152a
    B. Immunohistochemical analysis of paraffin-embedded melanoma using anti-CD146 RabMAb (Cat. #AJ1152a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P43121
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3208
Calculated MW 71607 Da
Gene ID 4162
Other Names Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, CD146, MCAM, MUC18
Target/Specificity A synthetic peptide corresponding to residues in human CD146 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD146 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MUC18
Function Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis
Research Areas
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CD146 (melanoma-associated antigen A32 or MUC18) is a transmembrane glycoprotein expressed on endothelial cells and is located at the intercellular junction where it plays a role in cell adhesion, and in the cohesion of the endothelial monolayer. CD146 engagement initiates an outside-in signaling pathway involving the protein tyrosine kinases FYN and FAK as well as Paxillin. In addition, it is a signaling molecule involved in the dynamics of actin cytoskeleton rearrangement (1). CD146 is associated with tumor progression and the development of metastasis in human malignant melanoma. Expression of the antigen is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage (2).


1. Anfosso F, et al. J Biol Chem. 276(2):1564-9, 2001
2. Sers C, et al. Proc Natl Acad Sci U S A. 90(18):8514-8, 1993

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Cat# AJ1152a
(40 western blots)
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