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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CD3 epsilon Antibody   

CD3 epsilon Antibody

Rabbit Monoclonal Antibody

  • WB - CD3 epsilon Antibody AJ1159a
    A. Western blot analysis of immunoprecipitation pellets from Jurkat cell lysate or 1XPBS (negative control) using anti-CD3 RabMAb (Cat. #AJ1159a), 1:50 dilution.
  • IHC - CD3 epsilon Antibody AJ1159a
    B. Immunohistochemical analysis of paraffin-embedded human lymph node using anti-CD3 RabMAb(Cat. #AJ1159a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07766
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names E272
Calculated MW 23147 Da
Gene ID 916
Other Names T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Target/Specificity A synthetic peptide corresponding to residues in C-terminal cytoplasmic domain of human CD-3 epsilon chain was used as immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD3 epsilon Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD3E
Synonyms T3E
Function The CD3 complex mediates signal transduction.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR) (1). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C (1,2). CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The gamma, epsilon and delta chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk (3).


1. Weiss, A., et al. Signal transduction by the T cell antigen receptor. Sem. Immunol. 3: 313
2. Siegel, J.N., et al. Multiple signal transduction pathways activated through the T cell receptor for antigen. Semin Immunol. 3: 325
3. Futterer, K., et al. Structural basis for Syk tyrosine kinase ubiquity in signal transduction pathways revealed by the crystal structure of its regulatory SH2 domains bound to a dually phosphorylated ITAM peptide. J Mol Biol. 281: 523

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Cat# AJ1159a
(40 western blots)
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