|Reactivity||Human, Mouse, Rat|
|Calculated MW||37408 Da|
|Other Names||Macrosialin, Gp110, CD68, CD68|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human CD68 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD68 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.|
|Cellular Location||Isoform Short: Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites|
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Provided below are standard protocols that you may find useful for product applications.
CD68 is a transmembrane glycoprotein of unknown function highly expressed by human monocytes and tissue macrophages. CD68 transcripts are absent or present at very low levels in many hematopoietic lines including KG1, CEM, and K562, but can be induced by exposure to phorbol myristic acid. The extracellular domain consists of two distinct regions separated by an extended proline hinge: a membrane-distal mucin-like domain containing short peptide repeats and consisting of 54% serine and threonine residues; and a membrane proximal domain that has significant sequence homology to a family of lysosomal/plasma membrane shuttling proteins known as the lamp 1 group. CD68 is a member of a growing family of hematopoietic mucin-like molecules, including leukosialin/CD43, the stem cell antigen CD34, and the lymph node high endothelial ligand for L-selectin GlyCAM-1 (1). Rapid recirculation of CD68 from endosomes, lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells (2).
1. Holness CL, et al. Blood 81(6):1607-13, 1993
2. Gerhard DS, et al. Genome Res 14(10B):2121-7, 2004
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