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CD99 Antibody

Rabbit Monoclonal Antibody

     
  • WB - CD99 Antibody AJ1173a
    A. Western blot analysis on (A) MOLT4, (B) Jurkat and (C) THP-1 cell lysates using anti-CD99 RabMAb (Cat. #AJ1173a), dilution 1:5,000.
  • IHC - CD99 Antibody AJ1173a
    B.Immunohistochemical analysis of paraffin-embedded human Ewing's sarcoma using anti-CD99 RabMAb.
  • IHC - CD99 Antibody AJ1173a
    C.Immunohistochemical analysis of paraffin-embedded human tonsils using anti-CD99 RabMAb (Cat. #AJ1173a).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P14209
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP14936-62
Calculated MW 18848 Da
Gene ID 4267
Other Names CD99 antigen, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, CD99, CD99, MIC2, MIC2X, MIC2Y
Target/Specificity A synthetic peptide corresponding to residues on the C-terminus of human CD99 was used as an immunogen.
Dilution WB~~1:1000~10000
IHC~~1:1000~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD99 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD99
Synonyms MIC2, MIC2X, MIC2Y
Function Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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Background

CD99, or protein MIC2, is a T-cell surface glycoprotein involved in the T-cell adhesion process and spontaneous rosette formation with erythrocytes. (1). The cell surface antigen defined by the monoclonal antibody CD99 is expressed by both the human X and Y chromosomes; the gene loci on the X and Y chromosomes are referred to as MIC2X and MIC2Y, respectively (2). It was demonstrated that engagement of distinct epitopes on CD99 rapidly induces T cell death by a novel caspase-independent pathway. Thus, it is suggested that CD99 is linked to a novel death pathway that may have biologic relevance in control of early T cells (3).

References

1. Gelin C, et al. EMBO J. 8(11):3253-9, 1989
2. Goodfellow P, et al. Am J Hum Genet. 36(4):777-82, 1984
3. Pettersen RD, et al. J Immunol. 166(8):4931-42, 2001

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Discontinued
Cat# AJ1173a
Size:
(40 western blots)
Alternative Products:

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